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Quantification of Escherichia coli via analysis of β-glucuronidase enzyme concentrations

dc.contributor.advisorMugo, Samuel
dc.contributor.authorAndersen, Brody
dc.date.accessioned2017-05-04
dc.date.accessioned2022-05-28T00:37:04Z
dc.date.available2022-05-28T00:37:04Z
dc.date.issued2017
dc.descriptionPresented on April 24, 2017 at Student Research Day held at MacEwan University in Edmonton, Alberta.
dc.description.abstractConcentration of Escherichia coli can be quantified based on a digestive enzyme produced by 97% of E. coli strains called β-glucuronidase (β-GUS). When in contact with a β-glucuronide (β-GLU) molecule, the enzyme cleaves the β-GLU segment off the molecule, leaving the remaining fragment untouched. The remaining fragment can serve as a marker for the presence of the enzyme and can be quantifiably calibrated to determine the concentration of the E. coli in each sample. For a colourimetric method approach, 4-nitrophenol-β-D-glucuronide (4-NβDg) can be used as a dye for the enzyme. The remainder of the molecule after enzymatic cleavage is a 4-nitrophenol, which is blue in colour. The change in colour can be quantified based on a calibration curve. For an electrochemical method approach, 4-NβDg can also be used because 4-nitrophenol gives a characteristic cyclic voltammogram on a potentiostat. The change in resistance of 4-nitrophenol can be determined and calibrated to show the concentration of the E. coli in each sample. This research is ongoing and does not have the finalized results on the outcome of the work described above. Additional information can be provided as necessary.
dc.format.extent709.2 KB
dc.format.mimetypePDF
dc.identifier.urihttps://hdl.handle.net/20.500.14078/847
dc.languageEnglish
dc.language.isoen
dc.relation.urihttp://roam.macewan.ca/islandora/object/gm:1331
dc.rightsAll Rights Reserved
dc.titleQuantification of Escherichia coli via analysis of β-glucuronidase enzyme concentrationsen
dc.typeStudent Creative Work
dspace.entity.type

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